Data-enriched characterization of cell populations. Morphological info from visuals can increase to your richness of flow cytometry knowledge. Such as, the figure reveals an if not traditional apoptosis assay making use of Annexin V and PI, including cell imaging to characterize cells in Every single inhabitants to expose morphologically distinctive functions. These insights could not have been received from multiplex staining alone.
As samples are obtained within the Attune CytPix Flow Cytometer, the significant-velocity brightfield digital camera captures and outlets photographs of detected functions in a price up to 6,000 photos per 2nd, dependant upon the flow fee and picture dimension. For bigger overall flexibility, Attune Cytometric Software package allows you to regulate the graphic capture frequency as required and choose a selected gate for image seize, if required.
Mix acoustic focusing technologies with higher-parameter analysis for adaptable spectral unmixing and traditional workflows.
The award-successful Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating which has a high-pace brightfield digicam for simultaneous large throughput flow cytometry and large resolution brightfield imaging.
"An image just isn't really worth a thousand dots, but it really genuinely helps to see photos. It is excellent to check out pictures that correlate with staining. As an example, AnnexinV-beneficial cells which are morphologically diverse than balanced cells, or CD14+ monocytes that happen to be larger and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
In this article, a highly skilled person has gated singlets confidently. Right after evaluating the guide singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate includes more than four% aggregates.
Optimizing gating strategy. Even robust manual singlet gating is mistake-vulnerable and continues to be a subjective final decision stage in Virtually all flow cytometry assays. Imaging may be used to confirm and regulate gates to include only solitary cells of fascination.
Dependable picture high-quality even at substantial throughput Acoustic concentrating and a superior-speed camera combine to graphic these T cells constantly at very low or large flow fees. Quickly alter focus and digicam options to fulfill experimental needs.
The Attune NxT cytometer will be the dependable workhorse instrument your lab can count on for consistent and trustworthy effectiveness. The Attune CytPix product contains a significant-pace brightfield digital camera and AI-driven picture Assessment software, with current upgrades which include machine learning instruments and an intuitive person interface, enabling enhanced knowledge precision, accuracy, and quicker insights into sample biology.
Possibly most of all, these activities have cells of clearly distinctive phenotypes which may lead to incorrect conclusions regarding double optimistic gatherings (especially in unusual populations).
AI-driven automatic graphic Examination with the Attune Cytometric Software package translates function capabilities into unique morphology parameters which can be combined with typical fluorescence and scatter parameters to expose very reproducible inhabitants variations and exceptional subsets.
We previously demonstrated the power of imaging Vehicle-T/Ramos mobile interactions. Allow’s have a look at just the populace of finest fascination, the double beneficial functions, to learn more. We could now use prolonged morphological parameters (below, circularity and skewness of depth) to even more look at the characteristics of such populations and refine gating on these activities, increasing data robustness.
Created-in dimensionality reduction instruments enhanced resolution into populations. Fluorescently gated populations back-gated on to UMAP plot to verify predicted population resolution in lysed full human website blood. Person can see functions from Each and every cluster for Visible affirmation of mobile phenotypes.
No clean/no lyse leukocyte Assessment with imaging. Cells had been obtained from 24-hour-old blood by dilution in 1 mM EDTA ( Professional UX/UI allows users to train AI styles for expanded purposes. (A) The “Teach Design” attribute gives annotation tools and authentic-time product teaching precision.
Gating on these extended parameters permits you to promptly and correctly establish populations of fascination and confirm gating tactic with little if any manual review. The Attune software package features use of condition-of-the-art device Studying Evaluation tools in an expertly designed user interface, which makes it exceptionally simple to use.
As the cell line had Formerly been analyzed to the Attune CytPix Flow Cytometer, the scientists went again to the photographs and have been capable to doc the microbial an infection at least 5 days earlier.
The Attune CytPix Flow Cytometer makes it possible for users to visualize samples through acquisition, verify the precision of gating methods, and aid results with responsible documentation for publication applications.
What appeared to be a homogeneous population is actually extra assorted—an insight that ought to be deemed when interpreting results.